Seminal fluid proteins differ in abundance between genetic lineages of honeybees

► We performed a DIGE analysis of seminal fluid between three genotypes of honeybees ► We compared the abundance of over 200 seminal fluid protein spots on 2D gels ► We found significant differences in seminal fluid protein abundance between genotypes ► We conclude that seminal fluid protein abundance is genotype specific ► Variation in seminal fluid protein abundance could result in natural/sexual selection