کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10559472 969627 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural and functional characterisation of a biohybrid material based on acetylcholinesterase and layered double hydroxides
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Structural and functional characterisation of a biohybrid material based on acetylcholinesterase and layered double hydroxides
چکیده انگلیسی
This work describes the use of layered double hydroxides (LDHs) for the immobilisation of acetylcholinesterase (AChE) on insulator/semiconductor solid supports. Different LDHs have been synthesised by a co-precipitation method. Afterwards, biohybrid materials based on AChE-LDH mixtures have been produced using wild and recombinant enzymes. Spectroscopic techniques have confirmed the LDH phase identity and the links created between the LDH and AChE. Spectrophotometric assays have demonstrated that most of the biohybrid materials are functional and stable. Several configurations have been used for AChE immobilisation. The highest catalytic responses have been observed when using wild enzyme and immobilising AChE-LDH mixtures on LDHs previously deposited on the solid supports. LDHs have been demonstrated to be suitable host matrices for AChE immobilisation on electrodes for the subsequent development of electrochemical biosensors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 85, Issue 4, 30 September 2011, Pages 1882-1887
نویسندگان
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