کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10559536 | 969642 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Covalent immobilization of horseradish peroxidase via click chemistry and its direct electrochemistry
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A simple and versatile approach for covalent immobilization of redox protein on solid surface via self-assembled technique and click chemistry is reported. The alkynyl-terminated monolayers are obtained by self-assembled technique, then, azido-horseradish peroxidase (azido-HRP) was covalent immobilized onto the formed monolayers by click reaction. The modified process is characterized by reflection absorption infrared spectroscopy (RAIR), surface-enhanced Raman scattering spectroscopy (SERS) and electrochemical methods. All the experimental results suggest that HRP is immobilized onto the electrode surface successfully without denaturation. Furthermore, the immobilized HRP shows electrocatalytic reduction for H2O2, and the linear range is from 5.0 to 700 μM. The heterogeneous electron transfer rate constant ks is 1.11 sâ1 and the apparent Michaelis-Menten constant is calculated to be 0.196 mM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 83, Issue 5, 15 February 2011, Pages 1381-1385
Journal: Talanta - Volume 83, Issue 5, 15 February 2011, Pages 1381-1385
نویسندگان
Qin Ran, Ru Peng, Cong Liang, Siqiu Ye, Yuezhong Xian, Wenjing Zhang, Litong Jin,