کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10559557 | 969642 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme](/preview/png/10559557.png)
چکیده انگلیسی
A novel affinity purification method for lysozyme (LZM) based on functionalized magnetic microspheres was developed. Tris(hydroxymethyl)aminomethane (Tris)-modified magnetic microspheres with specific affinity toward LZM were prepared using Tris as ligand and silica-coated magnetic microshperes as support. Transmission electron microscopy and magnetic property measurement results showed that the Tris-modified magnetic microspheres have a very good core-shell structure and high magnetization.The maximum binding capacity of LZM was about 108.6Â mg/g magnetic microspheres. LZM purified from chicken egg white had high purity and well-maintained activity of 8140Â U/mg. This magnetic-mediated LZM purification strategy has advantages of high efficiency, low cost and easy operation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 83, Issue 5, 15 February 2011, Pages 1515-1520
Journal: Talanta - Volume 83, Issue 5, 15 February 2011, Pages 1515-1520
نویسندگان
Guoqing Zhang, Qing Cao, Na Li, Kean Li, Feng Liu,