کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10560961 | 969759 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Carbon film electrodes for oxidase-based enzyme sensors in food analysis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Carbon film electrodes for oxidase-based enzyme sensors in food analysis Carbon film electrodes for oxidase-based enzyme sensors in food analysis](/preview/png/10560961.png)
چکیده انگلیسی
Carbon film resistor electrodes have been evaluated as transducers for the development of multiple oxidase-based enzyme electrode biosensors. The resistor electrodes were first modified with Prussian Blue (PB) and then covered by a layer of covalently immobilized enzyme. Electrochemical impedance spectroscopy was used to characterize the interfacial behaviour of the Prussian Blue modified and enzyme electrodes; the spectra demonstrated that the access of the substrates is essentially unaltered by application of the enzyme layer. These enzyme electrodes were used to detect the substrate of the oxidase (glucose, ethanol, lactate, glutamate) via reduction of hydrogen peroxide at +50Â mV versus Ag/AgCl in the low micromolar range. Response times were 1-2Â min. Finally, the glucose, ethanol and lactate electrochemical biosensors were used to analyse complex food matrices-must, wine and yoghurt. Data obtained by the single standard addition method were compared with a spectrophotometric reference method and showed good correlation, indicating that the electrodes are suitable for food analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 68, Issue 2, 15 December 2005, Pages 171-178
Journal: Talanta - Volume 68, Issue 2, 15 December 2005, Pages 171-178
نویسندگان
S. De Luca, M. Florescu, M.E. Ghica, A. Lupu, G. Palleschi, C.M.A. Brett, D. Compagnone,