A flow-injection renewable surface sensor for the fluorimetric determination of vanadium(V) with Alizarin Red S

The measurement of fluorescence is continuously performed at an excitation wavelength of 521 nm and an emission wavelength of 617 nm. Using a low sample volume of 800 μl, the analytical signal showed a very good linearity in the range 2-60 ng ml−1, with a detection limit of 0.45 ng ml−1 and a R.S.D. (%) of 4.22 for 50 ng ml−1 of V(V) concentration (n = 10). The sensor showed both a good selectivity, which could also be increased by the addition of EDTA and F− as masking agents, and applicability to the determination of V(V) in waters, physiological samples (serum and urine) and mussel tissues.