کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10561486 | 969775 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of the hydroxyl radical by its adduct formation with phenol and liquid chromatography/electrochemical detection
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
An HPLC-ECD method is described for the indirect determination of the hydroxyl (OH) radical. Fenton's reaction is used to produce OH, which simultaneously attacks phenols (phenol or pyrocatechol) to form the adducts, pyrocatechol or pyrogallic acid. Thus, [OH] quantification is based on the separation and detection of pyrogallic acid and/or pyrocatechol by an isocratic HPLC-ECD method. The quantification of OH is also performed alternatively by a chronoamperometric detection in an electrochemical cell, where simultaneously formed FeIII (Fenton's reaction) combines [FeII(CN)6]4â to produce the Prussian blue (PB) molecules (Fe4III[FeII(CN)6]3). Newly formed PB molecules are then immediately converted to colorless Everitts salt (K4Fe4II[FeII(CN)6]3) with the reduction of the high-spin FeIII to FeII at the surface of a glassy carbon electrode at +0.150Â V (versus Ag/AgCl). The calculated concentration of OH during incubation (0.626Â ppm) can be detected with negative errors by the HPLC-ECD (0.595 and 0.615Â ppm with the errors â5.2 and â1.8%, respectively) and by the chronoamperometric method (0.552 and 0.607Â ppm with the errors â11.8 and â3.0%, respectively). For the comparison of the two sets of data, HPLC-ECD method is much more promising.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 65, Issue 4, 28 February 2005, Pages 876-881
Journal: Talanta - Volume 65, Issue 4, 28 February 2005, Pages 876-881
نویسندگان
Emrah Kilinc,