Fluorescence anisotropy: application in quantitative enzymatic determinations

After optimisation, the proposed method allows the determination of glucose over the range 100-1000 mg l−1. The detection limit is 90 mg l−1and the reproducibility is better than 4% (n = 6, [glucose] = 250 mg l−1). Anisotropy is more selective than conventional fluorescence intensity, and this method has therefore been applied to direct glucose determination in fruit juices without the interference caused by the inner filter effect.