Effects of β-glucosidase hydrolyzed products of harpagide and harpagoside on cyclooxygenase-2 (COX-2) in vitro

The hydrolyzed products of harpagide (H-harpagide) with β-glucosidase treatment showed a significant inhibitory effect on COX-2 activity at 2.5-100 μM in a concentration-dependent manner. H-harpagide could bind to the COX-2 active domain well through hydrophobic and hydrogen-bonding interactions, whereas harpagide and harpagoside could not.