کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10586014 | 981383 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chemically modified siRNAs and miRNAs bearing urea/thiourea-bridged aromatic compounds at their 3â²-end for RNAi therapy
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
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چکیده انگلیسی
We have developed chemically modified siRNAs and miRNAs bearing urea/thiourea-bridged aromatic compounds at their 3â²-end for RNAi therapy. Chemically modified RNAs possessing urea/thiourea-bridged aromatic compounds instead of naturally occurring dinucleotides at the 3â²-overhang region were easily prepared in good yields and were more resistant to nucleolytic hydrolysis than unmodified RNA. siRNAs containing urea or thiourea derivatives showed the desired knockdown effect. Furthermore, modified miR-143 duplexes carrying the urea/thiourea compounds in the 3â²-end of each strand were able to inhibit the growth of human bladder cancer T24 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 21, Issue 15, 1 August 2013, Pages 4494-4501
Journal: Bioorganic & Medicinal Chemistry - Volume 21, Issue 15, 1 August 2013, Pages 4494-4501
نویسندگان
Yoshiaki Kitamura, Yuki Masegi, Shunsuke Ogawa, Remi Nakashima, Yukihiro Akao, Yoshihito Ueno, Yukio Kitade,