کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10587493 | 981430 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of rat synaptic membrane Na+/K+-ATPase and ecto-nucleoside triphosphate diphosphohydrolases by 12-tungstosilicic and 12-tungstophosphoric acid
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
12-tungstophosphoric acidSPMADPWPAPOMs - POM هاAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPadenosine diphosphate - آدنوزین دی فسفاتFourier transform infrared - تبدیل فوریه مادون قرمزFT-IR - طیف سنجی مادون قرمز تبدیل فوریهSynaptic plasma membrane - غشای پلاسمای سیناپتیکPolyoxometalates - پلی اکسومتالات ها
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The in vitro influence of Keggin structure polyoxotungstates, 12-tungstosilicic acid, H4SiW12O40 (WSiA) and 12-tungstophosphoric acid, H3PW12O40 (WPA), and monomer Na2WO4 Ã 2H2O on rat synaptic plasma membrane (SPM) Na+/K+-ATPase and E-NTPDase activity was studied, whereas the commercial porcine cerebral cortex Na+/K+-ATPase served as a reference. Dose-dependent Na+/K+-ATPase inhibition was obtained for all investigated compounds. Calculated IC50 (10 min) values, in mol/l, for SPM/commercial Na+/K+-ATPase, were: 3.4 Ã 10â6/4.3 Ã 10â6, 2.9 Ã 10â6/3.1 Ã 10â6 and 1.3 Ã 10â3/1.5 Ã 10â3 for WSiA, WPA and Na2WO4 Ã 2H2O, respectively. In the case of E-NTPDase, increasing concentrations of WSiA and WPA induced its activity reduction, while Na2WO4 Ã 2H2O did not noticeably affect the enzyme activity at all investigated concentrations (up to 1 Ã 10â3 mol/l). IC50 (10 min) values, obtained from the inhibition curves, were (in mol/l): 4.1 Ã 10â6 for WSiA and 1.6 Ã 10â6 for WPA. Monolacunary Keggin anion was found as the main active molecular species present under physiological conditions (in the enzyme assays, pH 7.4), for the both polyoxotungstates solutions (1 mmol/l), using Fourier transform infrared (FT-IR) and micro-Raman spectroscopy. Additionally, commercial porcine cerebral cortex Na+/K+-ATPase was exposed to the mixture of Na2WO4 Ã 2H2O and WSiA at different concentrations. Additive inhibition effect was achieved for lower concentrations of Na2WO4 Ã 2H2O/WSiA (⩽1 Ã 10â3/4 Ã 10â6 mol/l), while antagonistic effect was obtained for all higher concentrations of the inhibitors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 23, 1 December 2011, Pages 7063-7069
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 23, 1 December 2011, Pages 7063-7069
نویسندگان
Mirjana B. ÄoloviÄ, Danica V. Bajuk-BogdanoviÄ, NataÅ¡a S. AvramoviÄ, Ivanka D. Holclajtner-AntunoviÄ, Nada S. BoÅ¡njakoviÄ-PavloviÄ, Vesna M. VasiÄ, Danijela Z. KrstiÄ,