Microanalysis of oligosaccharide HS203 in beagle dog plasma by postcolumn fluorescence derivatization method

► A rapid and sensitive HPLC method was developed for oligosaccharide microanalysis. ► The plasma concentration-time curve of oligosaccharide HS203 was established. ► The blood glucose in beagle dog was monitored simultaneously using this method. ► This method is more sensitive than silver staining PAGE analysis of HS203. ► Our data is valuable for microanalysis of other sugars in biological samples.