Mutational analysis and characterization of dextran synthesizing enzyme from wild and mutant strain of Leuconostoc mesenteroides

► New strain of Leuconostoc mesenteroides was isolated. ► Mutagenesis was performed using UV method. ► An increased yield of dextransucrase was obtained from mutant strain. ► Vmax and Km values of wild strain changed after mutation. ► Wild and mutant strain had same molecular weight of 221 kDa and mutation did not changed the molecular weight.