Carbon nanotube-incorporated multilayered cellulose acetate nanofibers for tissue engineering applications

► Multilayers of CS/MWCNTs and CS/ALG can be successfully assembled onto the surface of CA nanofibers. ► Multilayer assembly of CS/MWCNTs and CS/ALG leads to increased surface roughness of the CA nanofibers. ► Incorporation of MWCNTs enables improved mechanical property of the multilayered CA fibrous scaffolds. ► Multilayered CA fibrous scaffolds incorporated with MWCNTs tend to promote fibroblast attachment, spreading, and proliferation. ► Hemocompatibility of CA nanofibers does not have significant changes after the assembly of multilayers.