کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10694484 | 1020120 | 2011 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of lunar and mars dust simulants on HaCaT keratinocytes and CHO-K1 fibroblasts
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
علوم فضا و نجوم
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![عکس صفحه اول مقاله: Effects of lunar and mars dust simulants on HaCaT keratinocytes and CHO-K1 fibroblasts Effects of lunar and mars dust simulants on HaCaT keratinocytes and CHO-K1 fibroblasts](/preview/png/10694484.png)
چکیده انگلیسی
Exposure to lunar dust during Apollo missions resulted in occasional reports of ocular, respiratory and dermal irritations which showed that lunar dust has a risk potential for human health. This is caused by its high reactivity as well as its small size, leading to a wide distribution also inside habitats. Hence, detailed information regarding effects of extraterrestrial lunar dusts on human health is required to best support future missions to moon, mars or other destinations. In this study, we used several methods to assess the specific effects of extraterrestrial dusts onto mammalian skin by exposing HaCaT keratinocytes and CHO-K1 fibroblasts to dusts simulating lunar or mars soils. These particular cell types were chosen because the skin protects the human body from potentially harmful substances and because a well orchestrated program ensures proper wound healing. Keratinocytes and fibroblasts were exposed to the dusts for different durations of time and their effects on morphology and viability of the cells were determined. Cytotoxicity was measured using the MTT assay and by monitoring culture impedance, while phalloidin staining of the actin cytoskeleton was performed to address structural integrity of the cells which was also investigated by propidium iodide intake. It was found that the effects of the two types of dust simulants on the different features of both cell lines varied to a considerable extent. Moreover, proliferation of HaCaT keratinocytes, as analyzed by Ki67 labeling, was suppressed in sub-confluent cultures exposed to lunar dust simulant. Furthermore, experimental evidence is provided for a delay in regeneration of keratinocyte monolayers from scratch-wounding when exposed to lunar dust simulant. The obtained results will facilitate further investigations of dust exposure during wound healing and will ease risk assessment studies e.g., for lunar lander approaches. The investigations will help to determine safety measures to be taken during extraterrestrial expeditions in order to minimize risks to human health associated with exposure of human skin to dust contaminants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Advances in Space Research - Volume 47, Issue 7, 1 April 2011, Pages 1200-1213
Journal: Advances in Space Research - Volume 47, Issue 7, 1 April 2011, Pages 1200-1213
نویسندگان
Maren Rehders, Bianka B. Grosshäuser, Anita Smarandache, Annapurna Sadhukhan, Ursula Mirastschijski, Jürgen Kempf, Matthias Dünne, Klaus Slenzka, Klaudia Brix,