کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738329 | 1046702 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
HSP70 mediates dissociation and reassociation of the 26S proteasome during adaptation to oxidative stress
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کلمات کلیدی
PBSMEFTCAtrichloroacetic acid - اسید ترشکلراکتیکStress adaptation - اقتباس تنشProtein oxidation - اکسیداسیون پروتئینProtein degradation - تخریب پروتئینOxidative stress - تنش اکسیداتیوFree radicals - رادیکال آزادubiquitin–proteasome system - سیستم ubiquitin-proteasomemurine embryonic fibroblasts - فیبروبلاست های جنینی جنینیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHemoglobin - هموگلوبین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We report an entirely new role for the HSP70 chaperone in dissociating 26S proteasome complexes (into free 20S proteasomes and bound 19S regulators), preserving 19S regulators, and reconstituting 26S proteasomes in the first 1-3 h after mild oxidative stress. These responses, coupled with direct 20S proteasome activation by poly(ADP ribose) polymerase in the nucleus and by PA28αβ in the cytoplasm, instantly provide cells with increased capacity to degrade oxidatively damaged proteins and to survive the initial effects of stress exposure. Subsequent adaptive (hormetic) processes (3-24 h after stress exposure), mediated by several signal transduction pathways and involving increased transcription/translation of 20S proteasomes, immunoproteasomes, and PA28αβ, abrogate the need for 26S proteasome dissociation. During this adaptive period, HSP70 releases its bound 19S regulators, 26S proteasomes are reconstituted, and ATP-stimulated proteolysis is restored. The 26S proteasome-dependent, and ATP-stimulated, turnover of ubiquitinylated proteins is essential for normal cell metabolism, and its restoration is required for successful stress adaptation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 51, Issue 7, 1 October 2011, Pages 1355-1364
Journal: Free Radical Biology and Medicine - Volume 51, Issue 7, 1 October 2011, Pages 1355-1364
نویسندگان
Tilman Grune, Betül Catalgol, Anke Licht, Gennady Ermak, Andrew M. Pickering, Jenny K. Ngo, Kelvin J.A. Davies,