کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738407 | 1046704 | 2011 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gold activates mast cells via calcium influx through multiple H2O2-sensitive pathways including L-type calcium channels
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کلمات کلیدی
DcfHAuCl4BMMCFcεRIACD2-aminoethoxydiphenyl borate2-APBCRACdichlorofluoresceincPLA2DCFH-DA[Ca2+]c - [Ca2 +] cBrown Norway - براون نروژAu(III) - در (III)Allergic contact dermatitis - درماتیت تماس آلرژیکdichlorodihydrofluorescein diacetate - دی کلستیدوفروفوروزین دی سکتهbone marrow-derived mast cell - سلول های مشتق شده از مغز استخوانendoplasmic reticulum - شبکه آندوپلاسمی cytosolic free Ca2+ concentration - غلظت Ca2 + آزاد بدون سیتوزولcytosolic phospholipase A2 - فسفولیپاز A2 سیتوزولAntigen - پادگِن یا آنتیژنhigh-affinity IgE receptor - گیرنده IgE باکتری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Heavy metals, including gold, induce severe contact hypersensitivity and autoimmune disorders, which develop through an initial Th2-independent process followed by a Th2-dependent process. It has been shown that mast cell activation plays a role in the Th2-independent process and that gold stimulates histamine release in vitro. However, the mechanisms of the gold-induced mast cell activation remain largely unclear. Here we report that gold directly activates mast cells in a Ca2+-dependent manner. HAuCl4 [Au(III)] at nontoxic concentrations (â¤Â 50 μM) induced substantial degranulation and leukotriene C4 secretion in an extracellular Ca2+-dependent manner. Au(III) induced a robust Ca2+ influx but not Ca2+ mobilization from internal stores. Au(III) also stimulated intracellular production of reactive oxygen species, including H2O2, and blockade of the production abolished the mediator release and Ca2+ influx. Au(III) induced Ca2+ influx through multiple store-independent Ca2+ channels, including Cav1.2 L-type Ca2+ channels (LTCCs) and 2-aminoethoxydiphenyl borate (2-APB)-sensitive Ca2+ channels. The 2-APB-sensitive channel seemed to mediate Au(III)-induced degranulation. Our results indicate that gold stimulates Ca2+ influx and mediator release in mast cells through multiple H2O2-sensitive Ca2+ channels including LTCCs and 2-APB-sensitive Ca2+ channels. These findings provide insight into the roles of these Ca2+ channels in the Th2-independent process of gold-induced immunological disorders.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 50, Issue 10, 15 May 2011, Pages 1417-1428
Journal: Free Radical Biology and Medicine - Volume 50, Issue 10, 15 May 2011, Pages 1417-1428
نویسندگان
Koremasa Hayama, Yoshihiro Suzuki, Toshio Inoue, Toyoko Ochiai, Tadashi Terui, Chisei Ra,