کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738737 | 1046751 | 2009 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mechanisms of stress resistance in Snell dwarf mouse fibroblasts: Enhanced antioxidant and DNA base excision repair capacity, but no differences in mitochondrial metabolism
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کلمات کلیدی
Snell dwarf miceAP EndonucleaseMnSODCATGPXFCCPCuZnSODapurinic/apyrimidinic endonuclease - apurinic / apyrimidinic endonucleaseROS - ROSHydrogen peroxide - آب اکسیژنهBase excision repair (BER) - تعمیر پایه برش (BER)Copper-zinc superoxide dismutase - سوپر اکسید دیسموتاز مس-رویmanganese superoxide dismutase - سوپر اکسید دیسموتاز منگنزCitrate synthase - سیترات سیتواستاتLifespan - طول عمرFibroblasts - فیبروبلاست هاLDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Serum deprivation - محرومیت سرمStress resistance - مقاومت در برابر استرسH2O2 - هیدروژن پراکسیدParaquat - پاراکواتCatalase - کاتالازcarbonylcyanide-p-trifluoromethoxyphenylhydrazone - کربونیل سایانیید-پتروفورورمتوکسفنیل هیدرازونglutathione peroxidase - گلوتاتیون پراکسیدازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dermal fibroblasts from long-lived Snell dwarf mice can withstand a variety of oxidative and non-oxidative stressors compared to normal littermate controls. Here, we report differences in the levels and activities of intracellular antioxidant and DNA repair enzymes between normal and Snell dwarf mice fibroblasts cultured under a variety of conditions, including: 3% and 20% ambient O2; the presence and absence of serum; and the addition of an exogenous oxidative stress. The only significant difference between normal and dwarf cells cultured in complete medium, at 20% O2, was an approximately 40% elevation of glutathione peroxidase (GPx) activity in the mutant cells. Serum deprivation elicited increases in GPx in both genotypes, but these activities remained higher in dwarf mouse cells. Dwarf mouse cells deprived of serum and challenged with exposure to paraquat or hydrogen peroxide showed a generally greater upregulation of catalase and DNA base excision repair enzymes. As these toxins can interact with mitochondria to increase mitochondrial ROS production, we explored whether there were differences in mitochondrial metabolism between normal and dwarf mouse cells. However, neither mitochondrial content nor the apparent mitochondrial membrane potential differed between genotypes. Overall, the results suggest that superior hydrogen peroxide metabolism and a marginally greater DNA base excision repair capacity contribute to the stress resistance phenotype of Snell dwarf mouse fibroblasts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 46, Issue 8, 15 April 2009, Pages 1109-1118
Journal: Free Radical Biology and Medicine - Volume 46, Issue 8, 15 April 2009, Pages 1109-1118
نویسندگان
Melissa M. Page, Adam B. Salmon, Scott F. Leiser, Ellen L. Robb, Melanie F. Brown, Richard A. Miller, Jeffrey A. Stuart,