کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738953 | 1046849 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Modification of endoplasmic reticulum Ca2+ stores by select oxidants produces changes reminiscent of those in cells from patients with Alzheimer disease
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کلمات کلیدی
BSSKMVRyRhypoxanthine/xanthine oxidaseDAF-FMNO−Presenilin-1S-nitroso-N-acetylpenicillamineInsP3RSIN-13-morpholinosydnonimine - 3-مورفولینویدونیمینیم4-Amino-5-Methylamino-2′,7′-Difluorofluorescein Diacetate - 4-آمینو-5-متیل آمینو-2 '، 7'-Difluorofluorescein diacetateFura-2AM - FURA-02:00NO+ - NO +O2− - O2-ROS - ROSt-BHP - T-BHP[Ca2+]i - [Ca2 +] iHydrogen peroxide - آب اکسیژنهNO nitric oxide - اکسید نیتریک NOOxidants - اکسیدان هاbradykinin - برادیکینینAlzheimer's disease - بیماری آلزایمرOxidative stress - تنش اکسیداتیوFree radicals - رادیکال آزادsuperoxide radical - رادیکال سوپراکسیدHydroxyl radical - رادیکال هیدروکسیلendoplasmic reticulum - شبکه آندوپلاسمی SNAP - ضربه محکم و ناگهانیcytosolic free calcium concentration - غلظت کلسیم رایگان سیتوزولFura-2-acetoxymethyl ester - فورا 2-اتوکسی متیل استرFibroblasts - فیبروبلاست هاbalanced salt solution - محلول نمک متعادلsodium nitroprusside - نیتروپروساید سدیمH2O2 - هیدروژن پراکسیدSNP - چندریختی تک-نوکلئوتیدReactive oxygen species - گونههای فعال اکسیژنInositol trisphosphate receptor - گیرنده inositol trisphosphateRyanodine receptor - گیرنده رایانودین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Abnormalities in calcium homeostasis and oxidative processes occur in fibroblasts from patients with Alzheimer disease (AD) and in fibroblasts and neurons from transgenic mice bearing a presenilin-1 (PS-1) mutation. Bombesin-releasable endoplasmic reticulum Ca2+ stores (BRCS) are exaggerated in all of these cells. Our previous studies show that H2O2 exaggerates BRCS. The goal of the present study was to determine whether select reactive species exaggerate BRCS in cultured human fibroblasts and to determine if the ability of fibroblasts to handle these specific oxidant species is altered in cells from AD patients. Two fluorescent indicators were used to distinguish different reactive oxygen species (ROS): 6-carboxy-2â²,7â²-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) (c-DCF) and 4-amino-5-methylamino-2â²,7â²-difluorofluorescein diacetate (DAF-FM). ROS were produced by a variety of oxidants, including tert-butyl-hydroxyperoxide (t-BHP), hypoxanthine/xanthine oxidase, S-nitroso-N-acetylpenicillamine, 3-morpholinosydnonimine, and sodium nitroprusside. Different oxidants selectively induced various ROS in distinct patterns. These oxidants also induced selective modification in [Ca2+]i and/or BRCS. Of the several oxidants tested, t-BHP was most specific for exaggerating BRCS without affecting basal [Ca2+]i and inducing only c-DCF-detectable ROS. On the other hand, the results show that NO that reacted with DAF-FM was not responsible for alterations in BRCS. Furthermore, the c-DCF-detectable ROS production induced by t-BHP was higher in fibroblasts from AD patients bearing a PS-1 mutation (n = 7) than in those from aged controls (n = 8). The higher production of c-DCF-detectable ROS may underlie the exaggeration of BRCS in fibroblasts from AD patients. Thus, these results are consistent with the hypothesis that abnormalities in selective cellular ROS cause AD-related changes in intracellular calcium regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 8, 15 October 2005, Pages 979-989
Journal: Free Radical Biology and Medicine - Volume 39, Issue 8, 15 October 2005, Pages 979-989
نویسندگان
Hsueh-Meei Huang, Huan-Lian Chen, Hui Xu, Gary E. Gibson,