کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738959 | 1046849 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Procyanidin B2 has anti- and pro-oxidant effects on metal-mediated DNA damage
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کلمات کلیدی
DTPAdiethylenetriamine-N,N,N′,N″,N″-pentaacetic acid8-oxodGHPLC-ECDESRFBSPBS8-oxo-7,8-dihydro-2′-deoxyguanosine - 8-اکسو-7،8-دی هیدرو-2'-دگزسی گوانوزینDNA damage - آسیبDNAIron - آهنFree radicals - رادیکال آزادElectron spin resonance - رزونانس اسپین الکترونSOD - سدfetal bovine serum - سرم جنین گاوSuperoxide dismutase - سوکسوکس دیسموتازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCopper - مسProcyanidin B2 - پروسیانیدین B2Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Procyanidin B2 (epicatechin-(4β-8)-epicatechin), which is present in grape seeds, apples, and cacao beans, has antioxidant properties. We investigated the mechanism of preventive action of procyanidin B2 against oxidative DNA damage in human cultured cells and isolated DNA. Procyanidin B2 inhibited the formation of 8-oxo-7,8-dihydro-2â²-deoxyguanosine (8-oxodG) in the human leukemia cell line HL-60 treated with an H2O2-generating system. In contrast, a high concentration of procyanidin B2 increased the formation of 8-oxodG in HL-60 cells. Experiments with calf thymus DNA also revealed that procyanidin B2 decreased 8-oxodG formation by Fe(II)/H2O2, whereas procyanidin B2 induced DNA damage in the presence of Cu(II), and H2O2 extensively enhanced it. An electron spin resonance spin trapping study utilizing 3,3,5,5-tetramethyl-1-pyrroline-N-oxide (M4PO) demonstrated that procyanidin B2 decreased the signal of M4PO-OH from H2O2 and Fe(II), whereas procyanidin B2 enhanced the signal from H2O2 and Cu(II). As an antioxidant mechanism, UV-visible spectroscopy showed that procyanidin B2 chelated Fe(II) at equivalent concentrations. As a pro-oxidant property, we examined DNA damage induced by procyanidin B2, using 32P-labeled DNA fragments obtained from genes relevant to human cancer. Our results raise the possibility that procyanidin B2 exerts both antioxidant and pro-oxidant properties by interacting with H2O2 and metal ions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 8, 15 October 2005, Pages 1041-1049
Journal: Free Radical Biology and Medicine - Volume 39, Issue 8, 15 October 2005, Pages 1041-1049
نویسندگان
Katsuhisa Sakano, Mika Mizutani, Mariko Murata, Shinji Oikawa, Yusuke Hiraku, Shosuke Kawanishi,