کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739022 | 1046853 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Measurement of S-nitrosothiols in extracellular fluids from healthy human volunteers and rheumatoid arthritis patients, using electron paramagnetic resonance spectrometry
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کلمات کلیدی
Rheumatoid arthritis - آرتریتروماتوئیدinflammation - التهاب( توروم) Human - انسانElectron paramagnetic resonance - تشدید پارامغناطیس الکترونFree radicals - رادیکال آزادsynovial fluid - مایع زلالهای، مایع مفصلی، مایع سینوویالgastric fluid - مایع معدهNitrate - نیتراتNitrosothiol - نیتروستیولNitrite - نیتریتNitric oxide - نیتریک اکسید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In human tissues, S-nitrosothiols (RSNOs) are generated by the nitric oxide (NO)-dependent S-nitrosation of thiol-containing species. Here, a novel electron paramagnetic resonance spectrometry assay for RSNOs is described, together with its application to studies of human health and disease. The assay involves degrading RSNOs using N-methyl-d-glucamine dithiocarbamate (MGD) at high pH and spin trapping the NO released using (MGD)2-Fe2+. Because dietary nitrate might contribute to tissue RSNOs, the assay was used to monitor the effect of Na15NO3 ingestion on plasma and gastric juice RSNOs in healthy human volunteers. Na15NO3 ingestion (2 mmol) increased gastric RS15NO concentrations (p < 0.01), but there was no significant effect on plasma RS15NO concentrations. Having established that dietary nitrate was not a confounding factor, we applied the RSNO assay to matched plasma and knee-joint synovial fluid (SF) from rheumatoid arthritis (RA) patients, with healthy subjects as controls. Clinical markers of RA inflammatory disease activity were quantified, as were plasma and SF NO2â and NO3â. Median RSNO concentrations were 0 (interquartile range 68) nM, 109 (282) nM, and 309 (470) nM in normal plasma, RA plasma, and SF, respectively. The median RSNO concentration was significantly elevated in RA SF compared with RA plasma (p < 0.05) and in RA plasma compared with normal plasma (p < 0.05). SF RSNO concentrations correlated positively with SF neutrophil counts (rs = 0.55, p < 0.05) and inversely with blood hemoglobin concentrations (rs = â0.52, p < 0.05), but not with NO2â or NO3â. Thus the raised levels of RSNOs in RA SF correlate with some established markers of inflammation, suggesting the described RSNO assay may have applications in rapid clinical monitoring of NO metabolism in human inflammatory conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 7, 1 October 2005, Pages 937-948
Journal: Free Radical Biology and Medicine - Volume 39, Issue 7, 1 October 2005, Pages 937-948
نویسندگان
Sophie A. Rocks, Claire A. Davies, Siobhán L. Hicks, Andrew J. Webb, Rainer Klocke, Graham S. Timmins, Atholl Johnston, Ali S.M. Jawad, David R. Blake, Nigel Benjamin, Paul G. Winyard,