کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739334 | 1046871 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic analysis of 4-hydroxy-2-nonenal-modified proteins in G93A-SOD1 transgenic mice-A model of familial amyotrophic lateral sclerosis
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کلمات کلیدی
5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazoliumDRP-2dihydropyrimidinase-related protein 2mSOD1SALSHNEPMSFhsp70SDSMALDI-TOFTCA3-[(3-cholamidopropyl)dimethylammonio]propanesulfonate - 3 - [(3-کلامیدوپروپیل) دی متیل آمونیو] پروپان سولفونات4-hydroxy-2-nonenal - 4-هیدروکسی-2 غیرنالsporadic ALS - ALS پراکندهamyotrophic lateral sclerosis - اسکلروز جانبی آمیوتروفیکtrichloroacetic acid - اسید ترشکلراکتیکAlzheimer's disease - بیماری آلزایمرALS - بیماری اسکلروز جانبی آمیوتروفیکOxidative stress - تنش اکسیداتیوNeurodegeneration - تولید نوروژنیکfALS - جعل اسنادMutant SOD1 - جهش SOD1Free radicals - رادیکال آزادmatrix-assisted laser desorption ionization-time of flight - زمان یونیزاسیون لیزر جذب لیزر ماتریس-زمان پروازsodium dodecyl sulfate - سدیم دودسیل سولفاتphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدLipid peroxidation - پراکسیداسیون لیپیدheat-shock protein 70 - پروتئین حرارت دهنده 70CHAPS - چاپس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Amyotrophic lateral sclerosis (ALS) is an age-related, fatal motor neuron degenerative disease occurring both sporadically (sALS) and heritably (fALS), with inherited cases accounting for approximately 10% of diagnoses. Although multiple mechanisms likely contribute to the pathogenesis of motor neuron injury in ALS, recent advances suggest that oxidative stress may play a significant role in the amplification, and possibly the initiation, of the disease. Lipid peroxidation is one of the several outcomes of oxidative stress. Since the central nervous system (CNS) is enriched with polyunsaturated fatty acids, it is particularly vulnerable to membrane-associated oxidative stress. Peroxidation of cellular membrane lipids or circulating lipoprotein molecules generates highly reactive aldehydes, among which is 4-hydroxy-2-nonenal (HNE). HNE levels are increased in spinal cord motor neurons of ALS patients, indicating that lipid peroxidation is associated with the motor neuron degeneration in ALS. In the present study, we used a parallel proteomic approach to identify HNE-modified proteins in the spinal cord tissue of a model of fALS, G93A-SOD1 transgenic mice, in comparison to the nontransgenic mice. We found three significantly HNE-modified proteins in the spinal cord of G93A-SOD1 transgenic mice: dihydropyrimidinase-related protein 2 (DRP-2), heat-shock protein 70 (Hsp70), and possibly α-enolase. These results support the role of oxidative stress as a major mechanism in the pathogenesis of ALS. Structural alteration and activity decline of functional proteins may consistently contribute to the neurodegeneration process in ALS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 7, 1 April 2005, Pages 960-968
Journal: Free Radical Biology and Medicine - Volume 38, Issue 7, 1 April 2005, Pages 960-968
نویسندگان
Marzia Perluigi, H. Fai Poon, Kenneth Hensley, William M. Pierce, Jon B. Klein, Vittorio Calabrese, Carlo De Marco, D. Allan Butterfield,