کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739542 | 1046879 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased cyclooxygenase-2 expression in peripheral blood mononuclear cells of smokers and hyperlipidemic subjects
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کلمات کلیدی
normolipidemicPBMCNSMoxLDLMMPCOX-2HDLhigh-density lipoprotein - HDL یا لیپوپروتئین با دانسیته بالا یا چگالی بالاOxidized LDL - LDL اکسید شدهinflammation - التهاب( توروم) Free radicals - رادیکال آزادperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیCyclooxygenase-2 - سیکلوکوکسیژناز2Smoking - سیگار کشیدنSmokers - سیگاری هاRisk factors - عوامل خطرNonsmokers - غیر سیگاری هاLow-density lipoprotein - لیپوپروتئین کم چگالی یا الدیال LDL - لیپوپروتئین کم چگالی(کلسترول بد)matrix metalloproteinase - ماتریکس متالوپروتئینازHyperlipoproteinemia - هیپرلیپوپروتئینمیHyperlipidemic - هیپرلیپیدمیHypercholesterolemia - هیپرکلسترولمی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Increased cyclooxygenase-2 expression in peripheral blood mononuclear cells of smokers and hyperlipidemic subjects Increased cyclooxygenase-2 expression in peripheral blood mononuclear cells of smokers and hyperlipidemic subjects](/preview/png/10739542.png)
چکیده انگلیسی
Cyclooxygenase (COX)-2 is expressed in macrophages of arteriosclerotic lesions and promotes inflammation. We investigated whether COX-2 is already expressed in peripheral blood mononuclear cells (PBMCs) of subjects possessing risk-related factors, such as in smokers and hyperlipidemics. PBMCs were isolated from the venous blood of normolipidemic nonsmokers (NL-NSM; n = 15), normolipidemic smokers (NL-SM; n = 12), hyperlipidemic nonsmokers (HL-NSM; n = 10), and hyperlipidemic smokers (HL-SM; n = 10). RNA from PBMCs was used for RT-PCR. Plasma concentrations of oxidized low-density lipoproteins (oxLDL) were measured by ELISA, those of glutamate and cystine by HPLC. The results show that COX-2 expression in PBMCs was significantly increased in the groups with cardiovascular risk factors (NL-SM, HL-SM, HL-NSM) compared with NL-NSM. COX-2 expression in PBMCs was positively correlated with concentrations of total serum cholesterol, oxLDL, glutamate, or cystine. We suggest that the elevated COX-2 expression indicates a priming of PBMCs as a response to a systemic pro-oxidative and proinflammatory shift in subjects with cardiovascular risk factors, which might also contribute to growth and instability of arteriosclerotic lesions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 2, 15 January 2005, Pages 235-242
Journal: Free Radical Biology and Medicine - Volume 38, Issue 2, 15 January 2005, Pages 235-242
نویسندگان
Gabriel A. Bonaterra, Wulf Hildebrandt, Anne Bodens, Roland Sauer, Klaus A. Dugi, Hans-Peter Deigner, Wulf Dröge, Jürgen Metz, Ralf Kinscherf,