کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10747270 | 1048927 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Increased expression of ADAM12 and ADAM17 genes in laser-capture microdissected breast cancers and correlations with clinical and pathological characteristics
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کلمات کلیدی
ΔΔCtQ-RT-PCRADAM12Tgf-αΔCTTACECLiHB-EGFADAM17EGFRGPiLCMDCISGAPDHTGF-βmRNAEGFIGFCDIDNA - DNA یا اسید دزوکسی ریبونوکلئیکFFPE - MEPS.D. - SD.ADAM - آدامdeoxyribonucleic acid - اسید deoxyribonucleicstandard deviation - انحراف معیارIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیHER2/neu - با HER2 / neuGene expression - بیان ژنTumor grade - درجه تومورmessenger ribonucleic acid - رسوب ریبونوکلئیک اسیدRIN - رینBreast cancers - سرطان سینهRNA Integrity Number - شماره یکپارچه RNAepidermal growth factor - عامل رشد اپیدرمیtumor growth factor beta - عامل رشد تومور بتاInsulin-like growth factor - فاکتور رشد مانند انسولینLaser-capture microdissection - لیزر جذب microdissectionformalin-fixed paraffin embedded - پارافین ثابت فرمالین تعبیه شده استInvasive lobular carcinoma - کارسینوم لوبولار مهاجمInvasive ductal carcinoma - کارسینوم مجرای تهاجمیglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازEstrogen receptor - گیرنده استروژنHuman epidermal growth factor receptor 2 - گیرنده عامل فاکتور رشد اپیدرمی انسان 2Epidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمالProgesterone receptor - گیرنده پروژسترون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Increased expression of ADAM12 and ADAM17 genes in laser-capture microdissected breast cancers and correlations with clinical and pathological characteristics Increased expression of ADAM12 and ADAM17 genes in laser-capture microdissected breast cancers and correlations with clinical and pathological characteristics](/preview/png/10747270.png)
چکیده انگلیسی
Expression of ADAMs was analyzed using quantitative reverse-transcription polymerase chain reaction in laser-capture microdissected specimens of breast cancers and corresponding non-neoplastic breast tissues from 92 patients. The proteins' expression was confirmed by immunohistochemistry. Significantly elevated amounts of ADAM12L, ADAM12S and ADAM17 transcripts were found in malignant breast cells compared with normal breast tissue and both ADAMs proteins showed moderate to strong immunoexpression in tumor cells and peritumoral fibroblasts. ADAM12L and ADAM12S expressions were correlated with age, younger patients having higher expression of ADAM12L and ADAM12S; ductal cancers had higher expression of ADAM12L compared with lobular types, whereas ADAM12S was higher expressed in lobular cancers; higher expressions were found for both ADAM12 and ADAM17 in HER2/neu positive and highly proliferative cancers. High-grade cancers showed significantly increased expression of ADAM17. Our study on laser-capture microdissected specimens confers motivation for future work on development of ADAM-selective inhibitors for treatment of breast cancers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 114, Issue 2, February 2012, Pages 131-139
Journal: Acta Histochemica - Volume 114, Issue 2, February 2012, Pages 131-139
نویسندگان
Diana Narita, Edward Seclaman, Sorin Ursoniu, Andrei Anghel,