کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10748286 | 1050270 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Complementary role of CNNM2 in sperm motility and Ca2+ influx during capacitation
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ca2+ plays a central role in the regulation of sperm motility. We recently reported an unexpected role of CNNM4, a Mg2+ transporter, in this process by demonstrating perturbed Ca2+ influx and gradual loss of motility of Cnnm4-deficient sperm. However, Cnnm4-deficient male mice were not entirely infertile, and a significant Ca2+ response was still observed in their sperm. In the present study, we generated Cnnm4-deficient mice harboring a non-functional Cnnm2 allele (Cnnm2Î), to examine whether CNNM2 compensates for the lost function of CNNM4 in sperm. Cnnm2+/Î; Cnnm4Î/Î mice were infertile, and no obvious histological abnormalities were noted in their testis and epididymis. Their sperm showed normal morphology, but became immotile much more rapidly than those from Cnnm4Î/Î mice. When capacitation was initiated using serum albumin application, a rapid increase of intracellular Ca2+ levels was observed in most wild-type sperm, but only about half of sperm from Cnnm4Î/Î mice exhibited a Ca2+ response, and the response rate was further reduced in sperm from Cnnm2+/Î; Cnnm4Î/Î mice. Thus, sperm motility and Ca2+ response were more severely affected in sperm from Cnnm2+/Î; Cnnm4Î/Î mice than in those from Cnnm4Î/Î mice, implicating CNNM2 in regulating these processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 474, Issue 3, 3 June 2016, Pages 441-446
Journal: Biochemical and Biophysical Research Communications - Volume 474, Issue 3, 3 June 2016, Pages 441-446
نویسندگان
Daisuke Yamazaki, Yosuke Funato, Haruhiko Miyata, Masahito Ikawa, Hiroaki Miki,