کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10748323 | 1050271 | 2016 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Singlet oxygen generation during the oxidation of L-tyrosine and L-dopa with mushroom tyrosinase
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Singlet oxygen generation during the oxidation of L-tyrosine and L-dopa with mushroom tyrosinase Singlet oxygen generation during the oxidation of L-tyrosine and L-dopa with mushroom tyrosinase](/preview/png/10748323.png)
چکیده انگلیسی
The generation of singlet oxygen during the oxidation of tyrosine and L-dopa using mushroom tyrosinase in a phosphate buffer (pH 7.4), the model of melanin synthesis in melanocytes, was examined. The reaction was performed in the presence of 2,2,6,6-tetramethyl-4-piperidone (4-oxo-TEMP), an acceptor of singlet oxygen and the electron spin resonance (ESR) of the spin adduct, 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy (4-oxo-TEMPO), was measured. An increase in the ESR signal attributable to 4-oxo-TEMPO was observed during the oxidation of tyrosine and L-dopa with tyrosinase, indicating the generation of singlet oxygen. The results suggest that 1O2 generation via tyrosinase-catalyzed melanin synthesis occurs in melanocyte.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 471, Issue 4, 18 March 2016, Pages 450-453
Journal: Biochemical and Biophysical Research Communications - Volume 471, Issue 4, 18 March 2016, Pages 450-453
نویسندگان
Akimitsu Miyaji, Masahiro Kohno, Yoshihiro Inoue, Toshihide Baba,