کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10748575 1050275 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana
چکیده انگلیسی
Methylesterase family member 16 (MES16) is an integral component of chlorophyll breakdown. It catalyzes the demethylation of fluorescent chlorophyll catabolite (FCC) and pheophorbide in vitro, and specifically demethylates FCC in vivo. Here we report the crystal structure of MES16 from Arabidopsis thaliana at 2.8 Å resolution. The structure confirm that MES16 is a member of the α/β-hydrolase superfamily with Ser-87, His-239, and Asp-211 as the catalytic triad. Our biochemical studies reveal that MES16 has esterase activity with methyl-indole acetic acid as the substrate, and the catalytically essential role of Ser-87 has been demonstrated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 474, Issue 1, 20 May 2016, Pages 226-231
نویسندگان
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