کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10749000 | 1050284 | 2016 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Crystal structure of YeaZ from Pseudomonas aeruginosa
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Crystal structure of YeaZ from Pseudomonas aeruginosa Crystal structure of YeaZ from Pseudomonas aeruginosa](/preview/png/10749000.png)
چکیده انگلیسی
The Pseudomonas aeruginosa PA3685 locus encodes a conserved protein that shares 49% sequence identity with Escherichia coli YeaZ, which was recently reported as involved in the biosynthesis of threonylcarbamoyl adenosine (t6A), a universal modified tRNA nucleoside. Many YeaZ orthologues were reported as “essential for life” among various bacterial species, suggesting a critical role for both these proteins and for the t6A biosynthetic pathway. We provide here evidences that PA3685 protein (PaYeaZ) is essential. Additionally, we describe its purification, crystallization, and crystallographic structure. The crystal structure shows that PaYeaZ is composed of two domains one of which is the platform to form protein-protein interaction involved either in homodimeric assembly or in the formation of the multiprotein complex required for the synthesis of t6A. These features make the PaYeaZ protein a potential target candidate for the design of novel inhibitors able to hinder the complex formation and expected to abolish the crucial activity of t6A synthesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 470, Issue 2, 5 February 2016, Pages 460-465
Journal: Biochemical and Biophysical Research Communications - Volume 470, Issue 2, 5 February 2016, Pages 460-465
نویسندگان
Davide Vecchietti, Silvia Ferrara, Ruggero Rusmini, Raffaella Macchi, Mario Milani, Giovanni Bertoni,