کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10749283 | 1050287 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
New synthetic peptide with efficacy for heparin reversal and low toxicity and immunogenicity in comparison to protamine sulfate
ترجمه فارسی عنوان
پپتید مصنوعی جدید با کارایی برای تغییر هپارین و کمبود سمیت و ایمنی در مقایسه با سولفات پروتامین
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کلمات کلیدی
پروتامین، خنثی سازی هپارین، سمیت پروتامین، ایمنی زا واکنش پذیری متقابل،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Protamine sulfate (PS), the only clinically approved antidote to unfractionated heparin (UFH), is widely used for cardiopulmonary surgery or other extracorporeal circulation situations. However, the applications of PS have accompanied various severe side-effects. In this study, we presented a novel synthesized peptide compound (RRRRR-RRRRR-RRRRR-sulfate, R15S) served as a safer UFH antidote. Comparison studies were conducted between PS and R15S on efficacy and safety, aided by heparin neutralization studies, drug toxicity studies and anaphylactic analysis. Our research demonstrated that R15S contained comparable UFH neutralization activity in vitro and in rats in vivo as to active partial thromboplastin time (APTT) and anti-FXa assays. There was no cytotoxicity for R15S at 60 μg mgâ1 or below and the median lethal dose (LD50) of R15S in mice was 35.4 mg kgâ1, both of which were similar to that of PS. Furthermore, R15S exhibited no immunogenicity while it was obvious in guinea pigs immunized with PS. The level of cross-reactivity to anti-PS antibodies of R15S was about 30%. Both of which indicated much safer properties of R15S than PS. In conclusion, we presented a promising candidate R15S for UFH reversal, which is effective in neutralizing UFH and potentially safer in use.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 467, Issue 3, 20 November 2015, Pages 497-502
Journal: Biochemical and Biophysical Research Communications - Volume 467, Issue 3, 20 November 2015, Pages 497-502
نویسندگان
Tong Li, Zhiyun Meng, Xiaoxia Zhu, Hui Gan, Ruolan Gu, Zhuona Wu, Jian Li, Ying Zheng, Taoyun Liu, Peng Han, Su Han, Guifang Dou,