کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10749668 | 1050294 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The third to fifth zinc fingers play an essential role in the binding of ZFP809 to the MLV-derived PBS
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کلمات کلیدی
EMSAMoMLVNuRDERVstris-buffered saline-TweenHP1PBSLTReSETTBSTPVDFElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزRetroviral vector - بردار RetroviralLong terminal repeat - تکرار طولانی مدتEndogenous retroviruses - رتروویروسهای آندوژنیکPrimer-binding site - سایت پیوند پیوندCMV - سیتومگالوویروسHuman cytomegalovirus - سیتومگالوویروس انسانیMoloney murine leukemia virus - ویروس لوئی موش Moloneyheterochromatin protein 1 - پروتئین هتروکروماتین 1Polyvinylidene fluoride - پلی وینیلیدین فلوراید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Members of the kruppel-associated box-containing zinc finger protein (KRAB-ZFP) family mediate a number of cellular processes through binding to target DNA sequences via zinc fingers. Generally, zinc fingers recognize three-nucleotide sequences; however, this rule is not universally applicable. Zinc finger protein 809 (ZFP809) belongs to the KRAB-ZFP family and functions in repressing the expression of Moloney murine leukemia virus (MoMLV) via sequence-specific binding to the primer-binding site (PBS) located downstream of the MoMLV-long terminal repeat (LTR) and the induction of epigenetic modifications at LTR, such as repressive histone modifications and de novo DNA methylation. Previously, we demonstrated the role of the first to fifth zinc fingers of ZFP809 in binding to MLV PBS, indicating these zinc fingers do not recognize MLV PBS as a three-nucleotide sequence. Therefore, in the present study, we constructed truncated and mutated zinc fingers and examined their ability to bind to MLV PBS. The third to fifth zinc fingers of ZFP809 were found to be essential for binding to MLV PBS. Furthermore, the results of the present study indicate that other zinc fingers, which were not directly involved in binding to MLV PBS, may function in potentiating binding and stable protein expression. Further characterization of the amino acid sequences of zinc fingers will help further elucidate the functions and features of KRAB-ZFP and other zinc finger proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 469, Issue 3, 15 January 2016, Pages 490-494
Journal: Biochemical and Biophysical Research Communications - Volume 469, Issue 3, 15 January 2016, Pages 490-494
نویسندگان
Yu Ichida, Yuko Utsunomiya, Masafumi Onodera,