کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10750077 | 1050297 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of the subcellular localization of the human histone methyltransferase SETDB1
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کلمات کلیدی
nESSETDB1H3K9LMBhMTCrm1NLSeGFPmAbMonoclonal antibody - آنتی بادی مونوکلونالnuclear export signal - سیگنال صادرات هسته ایnuclear localization signal - سیگنال محلی سازی هسته ایLeptomycin B - لپتومایسین BProtein localization - محلی سازی پروتئینchromosome region maintenance 1 - نگهداری منطقه کروموزوم 1Histone Methyltransferase - هیستون متیل ترانسفرازenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته است
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
SET domain, bifurcated 1 (SETDB1) is a histone methyltransferase that methylates lysine 9 on histone H3. Although it is important to know the localization of proteins to elucidate their physiological function, little is known of the subcellular localization of human SETDB1. In the present study, to investigate the subcellular localization of hSETDB1, we established a human cell line constitutively expressing enhanced green fluorescent protein fused to hSETDB1. We then generated a monoclonal antibody against the hSETDB1 protein. Expression of both exogenous and endogenous hSETDB1 was observed mainly in the cytoplasm of various human cell lines. Combined treatment with the nuclear export inhibitor leptomycin B and the proteasome inhibitor MG132 led to the accumulation of hSETDB1 in the nucleus. These findings suggest that hSETDB1, localized in the nucleus, might undergo degradation by the proteasome and be exported to the cytosol, resulting in its detection mainly in the cytosol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 465, Issue 4, 2 October 2015, Pages 725-731
Journal: Biochemical and Biophysical Research Communications - Volume 465, Issue 4, 2 October 2015, Pages 725-731
نویسندگان
Keisuke Tachibana, Eiko Gotoh, Natsuko Kawamata, Kenji Ishimoto, Yoshie Uchihara, Hiroko Iwanari, Akira Sugiyama, Takeshi Kawamura, Yasuhiro Mochizuki, Toshiya Tanaka, Juro Sakai, Takao Hamakubo, Tatsuhiko Kodama, Takefumi Doi,