کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10751060 | 1050306 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Delivery of human NKG2D-IL-15 fusion gene by chitosan nanoparticles to enhance antitumor immunity
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کلمات کلیدی
DLSPBSIL-15NKG2DELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاTumor - تومورDegree of deacetylation - درجه آلودگیphosphate buffer saline - فسفات بافر شورMica - میکاNanoparticle - نانوذارتChitosan nanoparticle - نانوذرات کیتوزانGene vaccine - واکسن ژنیDynamic Light Scattering - پراکندگی نور دینامیکی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Nanoparticles are becoming promising carriers for gene delivery because of their high capacity in gene loading and low cell cytotoxicity. In this study, a chitosan-based nanoparticle encapsulated within a recombinant pcDNA3.1-dsNKG2D-IL-15 plasmid was generated. The fused dsNKG2D-IL-15 gene fragment consisted of double extracellular domains of NKG2D with IL-15 gene at downstream. The average diameter of the gene nanoparticles ranged from 200 nm to 400 nm, with mean zeta potential value of 53.8 ± 6.56 mV. The nanoparticles which were loaded with the dsNKG2D-IL-15 gene were uptaken by tumor cells with low cytotoxicity. Tumor cells pre-transfected by gene nanopartilces stimulated NK and T cells in vitro. Intramuscular injection of gene nanoparticles suppressed tumor growth and prolonged survival of tumor-bearing mice through activation of NK and CD8+ T cells. Thus, chitosan-based nanoparticle delivery of dsNKG2D-IL-15 gene vaccine can be potentially used for tumor therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 463, Issue 3, 31 July 2015, Pages 336-343
Journal: Biochemical and Biophysical Research Communications - Volume 463, Issue 3, 31 July 2015, Pages 336-343
نویسندگان
Chen Yan, Leng Jie, Wang Yongqi, Xiao Weiming, Xi Juqun, Ding Yanbing, Qian Li, Pan Xingyuan, Ji Mingchun, Gong Weijuan,