کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10751060 | 1050306 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Delivery of human NKG2D-IL-15 fusion gene by chitosan nanoparticles to enhance antitumor immunity
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کلمات کلیدی
DLSPBSIL-15NKG2DELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاTumor - تومورDegree of deacetylation - درجه آلودگیphosphate buffer saline - فسفات بافر شورMica - میکاNanoparticle - نانوذارتChitosan nanoparticle - نانوذرات کیتوزانGene vaccine - واکسن ژنیDynamic Light Scattering - پراکندگی نور دینامیکی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Delivery of human NKG2D-IL-15 fusion gene by chitosan nanoparticles to enhance antitumor immunity Delivery of human NKG2D-IL-15 fusion gene by chitosan nanoparticles to enhance antitumor immunity](/preview/png/10751060.png)
چکیده انگلیسی
Nanoparticles are becoming promising carriers for gene delivery because of their high capacity in gene loading and low cell cytotoxicity. In this study, a chitosan-based nanoparticle encapsulated within a recombinant pcDNA3.1-dsNKG2D-IL-15 plasmid was generated. The fused dsNKG2D-IL-15 gene fragment consisted of double extracellular domains of NKG2D with IL-15 gene at downstream. The average diameter of the gene nanoparticles ranged from 200 nm to 400 nm, with mean zeta potential value of 53.8 ± 6.56 mV. The nanoparticles which were loaded with the dsNKG2D-IL-15 gene were uptaken by tumor cells with low cytotoxicity. Tumor cells pre-transfected by gene nanopartilces stimulated NK and T cells in vitro. Intramuscular injection of gene nanoparticles suppressed tumor growth and prolonged survival of tumor-bearing mice through activation of NK and CD8+ T cells. Thus, chitosan-based nanoparticle delivery of dsNKG2D-IL-15 gene vaccine can be potentially used for tumor therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 463, Issue 3, 31 July 2015, Pages 336-343
Journal: Biochemical and Biophysical Research Communications - Volume 463, Issue 3, 31 July 2015, Pages 336-343
نویسندگان
Chen Yan, Leng Jie, Wang Yongqi, Xiao Weiming, Xi Juqun, Ding Yanbing, Qian Li, Pan Xingyuan, Ji Mingchun, Gong Weijuan,