کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10751635 | 1050317 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The small GTPase Rab33A participates in regulation of amylase release from parotid acinar cells
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کلمات کلیدی
LAMP1EGTAGSTBasolateral plasma membraneRT-PCRIPRDAPICytoSGMBLMVAMP2ICMAPMParotid6-diamidino-2-phenylindole - 6-دیامیدین-2-فنیلینولAmylase release - آزادی آمیلازStreptolysin O - استرپتولیسین Oslo - اسلوisoproterenol - ایزوپروترنولIntracellular distribution - توزیع داخل سلولیCytosol - سیتوزولApical plasma membrane - غشای پلاسمای اپیکالreverse transcription-PCR - معکوس رونویسی PCRVesicle-associated membrane protein 2 - پروتئین غشاء مرتبط با Vesicle 2lysosome-associated membrane protein 1 - پروتئین غشاء مرتبط با لیزوزوم 1glutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Amylase is released from exocrine parotid acinar cells via typical exocytosis. Exocytosis of amylase-containing granules occurs through several steps, including formation, maturation, and transport of granules. These steps are thought to be regulated by members of the small GTPase Rab family. We previously demonstrated that Rab27 and its effectors mediate amylase release from parotid acinar cells, but the functional involvement of other Rab proteins in exocrine granule exocytosis remains largely unknown. Here, we studied isoproterenol (IPR)-induced amylase release from parotid acinar cells to investigate the possible involvement of Rab33A, which was recently suggested to regulate exocytosis in hippocampal neurons and PC12 cells. Rab33A was endogenously expressed in parotid acinar cells and present in secretory granules and the Golgi body. Functional ablation of Rab33A with anti-Rab33A antibody or a dominant-negative Rab33A-T50N mutant significantly reduced IPR-induced amylase release. Our results indicated that Rab33A is a novel component of IPR-stimulated amylase secretion from parotid acinar cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 461, Issue 3, 5 June 2015, Pages 469-474
Journal: Biochemical and Biophysical Research Communications - Volume 461, Issue 3, 5 June 2015, Pages 469-474
نویسندگان
Akane Imai, Maiko Tsujimura, Sumio Yoshie, Mitsunori Fukuda,