کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10752255 | 1050324 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of the moss PpLEA4-20 gene in rice enhances membrane protection and client proteins stability
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کلمات کلیدی
LEA2-DETOFLiquid chromatographIPG - GPAimmobilized pH gradient - gradient pH immobilizedRice - برنجmatrix-assisted laser desorption/ionization - جذب / یونیزاسیون لیزر ماتریس کمک می کندMembrane protection - حفاظت غشاءTransformation - دگرگونیtime of flight - زمان پروازmass spectrometer - طیف سنج جرمیMALDI - مالدیpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Green vegetative tissues of the moss Physcomitrella patens possess a powerful ability to tolerate severe drought stress. Proteomics analysis have revealed that a large number of late embryogenesis abundant (LEA) proteins were key players in the drought tolerance of the photosynthetic tissues. PpLEA4-20, a member of the moss LEA protein family, was selected for further function study using an ectopic expression method in rice. Through molecular identification via PCR, southern blotting and TAIL-PCR, we demonstrated that the PpLEA4-20 gene was transformed and inserted into a non-encoded region in chromosome 4 of rice and expressed stably in transgenic rice. Unexpectedly, PpLEA4-20 protein emerged as two high-expressed spots on 2-D gels generated from transgenic rice, suggesting that PpLEA4-20 proteins are complete compatible and might be modified in rice. Both growth and physiological analysis showed that seedlings of transgenic PpLEA4-20 rice displayed altered phenotypes and tolerance to salt. In addition, electrolyte leakage was reduced in transgenic PpLEA4-20 compared to wild type under stress conditions. Anti-aggregation analysis found that the PpLEA4-20 protein expressed in rice remained soluble at high temperature and in addition to some native proteins from transgenic PpLEA4-20 rice. Based on Nano LC MS/MS analysis, we identified several proteins from transgenic PpLEA4-20 rice of increased heat-stability. Our results provide evidence for a role of PpLEA4-20 in salt tolerance and stabilization of client proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 460, Issue 2, 1 May 2015, Pages 386-391
Journal: Biochemical and Biophysical Research Communications - Volume 460, Issue 2, 1 May 2015, Pages 386-391
نویسندگان
Li Li, Dandan Deng, Xi Chen, Baomei Wu, Ke Hu, Tianhang Qiu, Suxia Cui, Fang Huang,