کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10752295 | 1050324 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The elastic free energy of a tandem modular protein under force
ترجمه فارسی عنوان
انرژی آزاد انعطاف پذیر از یک پروتکل مدولار دو طرفه تحت نیروی
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کلمات کلیدی
پروتکل مدولار دو طرفه، چشم انداز انرژی آزاد، طیف سنجی نیروی، کشش بافتی، پروتئین تاشو، دینامیک لانگوین،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Recent studies have provided a theoretical framework for including entropic elasticity in the free energy landscape of proteins under mechanical force. Accounting for entropic elasticity using polymer physics models has helped explain the hopping behavior seen in single molecule experiments in the low force regime. Here, we expand on the construction of the free energy of a single protein domain under force proposed by Berkovich et al. to provide a free energy landscape for N tandem domains along a continuous polypeptide. Calculation of the free energy of individual domains followed by their concatenation provides a continuous free energy landscape whose curvature is dominated by the worm-like chain at forces below 20 pN. We have validated our free energy model using Brownian dynamics and reproduce key features of protein folding. This free energy model can predict the effects of changes in the elastic properties of a multidomain protein as a consequence of biological modifications such as phosphorylation or the formation of disulfide bonds. This work lays the foundations for the modeling of tissue elasticity, which is largely determined by the properties of tandem polyproteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 460, Issue 2, 1 May 2015, Pages 434-438
Journal: Biochemical and Biophysical Research Communications - Volume 460, Issue 2, 1 May 2015, Pages 434-438
نویسندگان
Jessica Valle-Orero, Edward C. Eckels, Guillaume Stirnemann, Ionel Popa, Ronen Berkovich, Julio M. Fernandez,