کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10752621 | 1050329 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
b-series gangliosides crucially regulate leptin secretion in adipose tissues
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کلمات کلیدی
DMEMFBSGD3 synthaseSVFGEMMβCDRT-PCRGangliosideBATDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoAdipose tissue - بافت چربیWhite adipose tissue - بافت چربی سفیدbrown adipose tissue - بافت چربی قهوه ایCNS - دستگاه عصبی مرکزیfetal bovine serum - سرم جنین گاوcentral nervous system - سیستم عصبی مرکزیLipid raft - قایق های چربLeptin - لپتین methyl-β-cyclodextrin - متیل-β-سیکلوکودکسترینknockout - ناکاوتwild type - نوع وحشیreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسWAT - چی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Gangliosides are widely involved in the regulation of cells and organs. However, little is known about their roles in leptin secretion from adipose tissues. Genetic deletion of b-series gangliosides resulted in the marked reduction of serum leptin. Expression analysis of leptin revealed that leptin accumulated in the adipose tissues of GD3 synthase-knockout (GD3S KO) mice. Analysis of primary cultured stromal vascular fractions (SVF) derived from GD3S KO mice revealed that leptin secretion was reduced, although leptin amounts in cells were increased compared with those of wild type. Interestingly, addition of b-series gangliosides to the culture medium of differentiated SVF resulted in the restoration of leptin secretion. Results of methyl-β-cyclodextrin treatment of differentiated 3T3-L1 cells as well as immunocytostaining of leptin and caveolin-1 suggested that b-series gangliosides regulate the leptin secretion from adipose tissues in lipid rafts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 459, Issue 2, 3 April 2015, Pages 189-195
Journal: Biochemical and Biophysical Research Communications - Volume 459, Issue 2, 3 April 2015, Pages 189-195
نویسندگان
Shuting Ji, Yuki Ohkawa, Kyohei Tokizane, Yuhsuke Ohmi, Ryoichi Banno, Keiko Furukawa, Hiroshi Kiyama, Koichi Furukawa,