کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10753188 | 1050335 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Compensatory hyperinsulinemia in high-fat diet-induced obese mice is associated with enhanced insulin translation in islets
ترجمه فارسی عنوان
هیپرانسولینمی عضلانی در موش های چاق مبتلا به رژیم غذایی با چربی با افزایش ترجمه انسولین در جزایر همراه است
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کلمات کلیدی
MafAPdx1AHAPALT2DMPBSKRbOGTTCHXPancreatic and duodenal homeobox 1 - homeobox پانکراس و دوازدههinsulin tolerance test - آزمون تحمل انسولینOral glucose tolerance test - آزمون تحمل گلوکز خوراکیITT - اینجاType 2 diabetes mellitus - دیابت نوع دوRadioisotope - رادیوایزوتوپhigh-fat diet - رژیم غذایی با چربی بالاcycloheximide - سیکلوهایسیمیدpalmitate - طلاییPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریnormal chow - چای طبیعیhigh-fat - چربی بالا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
A high-fat diet (HF) is associated with obesity, insulin resistance, and hyperglycemia. Animal studies have shown compensatory mechanisms in pancreatic β-cells after high fat load, such as increased pancreatic β-cell mass, enhanced insulin secretion, and exocytosis. However, the effects of high fat intake on insulin synthesis are obscure. Here, we investigated whether insulin synthesis was altered in correlation with an HF diet, for the purpose of obtaining further understanding of the compensatory mechanisms in pancreatic β-cells. Mice fed an HF diet are obese, insulin resistant, hyperinsulinemic, and glucose intolerant. In islets of mice fed an HF diet, more storage of insulin was identified. We analyzed insulin translation in mouse islets, as well as in INS-1 cells, using non-radioisotope chemicals. We found that insulin translational levels were significantly increased in islets of mice fed an HF diet to meet systemic demand, without altering its transcriptional levels. Our data showed that not only increased pancreatic β-cell mass and insulin secretion but also elevated insulin translation is the major compensatory mechanism of pancreatic β-cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 458, Issue 3, 13 March 2015, Pages 681-686
Journal: Biochemical and Biophysical Research Communications - Volume 458, Issue 3, 13 March 2015, Pages 681-686
نویسندگان
Ayumi Kanno, Shun-ichiro Asahara, Katsuhisa Masuda, Tomokazu Matsuda, Maki Kimura-Koyanagi, Susumu Seino, Wataru Ogawa, Yoshiaki Kido,