کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10753274 | 1050338 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ascorbic acid transport in brain microvascular pericytes
ترجمه فارسی عنوان
انتقال اسید آسکوربیک در مغزهای مغز و اعصاب مغز
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کلمات کلیدی
Krebs-Ringer HEPESHEPESDAPING2GLUTPericytessodium-dependent vitamin C transporterRAGESVCT2dehydroascorbateKRHSVCT4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولN-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid - N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acidAscorbic acid - آسکوربیک اسیدTransport kinetics - جنبش حمل و نقلDHA - دوکوساهگزائنوئیک اسیدfacilitative glucose transporter - گاو نرReceptor for advanced glycation end products - گیرنده برای محصولات پیشرفته glycation پایان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Intracellular vitamin C, or ascorbic acid, has been shown to prevent the apoptosis of cultured vascular pericytes under simulated diabetic conditions. We sought to determine the mechanism by which ascorbate is transported into pericytes prior to exerting this protective effect. Measuring intracellular ascorbate, we found that pericytes display a linear uptake over 30 min and an apparent transport Km of 21 μM, both of which are consistent with activity of the Sodium-dependent Vitamin C Transporter 2 (SVCT2). Uptake of both radiolabeled and unlabeled ascorbate was prevented by inhibiting SVCT2 activity, but not by inhibiting the activity of GLUT-type glucose transporters, which import dehydroascorbate to also generate intracellular ascorbate. Likewise, uptake of dehydroascorbate was prevented with the inhibition of GLUTs, but not by inhibiting the SVCT2, indicating substrate specificity of both transporters. Finally, presence of the SVCT2 in pericytes was confirmed by western blot analysis, and immunocytochemistry was used to localize it to the plasma membrane and intracellular sites. Together, these data clarify previous inconsistencies in the literature, implicate SVCT2 as the pericyte ascorbate transporter, and show that pericytes are capable of concentrating intracellular ascorbate against a gradient in an energy- and sodium-dependent fashion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 458, Issue 2, 6 March 2015, Pages 262-267
Journal: Biochemical and Biophysical Research Communications - Volume 458, Issue 2, 6 March 2015, Pages 262-267
نویسندگان
William H. Parker, Zhi-chao Qu, James M. May,