کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10753956 | 1050345 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human glutathione S-transferase P1-1 functions as an estrogen receptor α signaling modulator
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
4-hydroxytamoxifenGlutathione S-transferase P1-14-OHTGSTPSERMERαTBSTEREPBSPVDFIgGDMSO - DMSOimmunoglobulin G - ایمونوگلوبولین Ganalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceDimethylsulfoxide - دیمتیل سولفواکسیدBreast cancer - سرطان پستانestrogen response element - عنصر پاسخ استروژنPhosphate buffered saline - فسفات بافر شورactivating protein - فعال سازی پروتئینactivating function - فعال کردن عملکردSelective estrogen receptor modulators - مدولاتور گیرنده استروژن انتخابیRIP - پاره کردنspecificity protein - پروتئین اختصاصیPoly vinylidene fluoride - پلی وینیلیدین فلورایدEstrogen receptor α - گیرنده استروژن α
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Estrogen receptor α (ERα) plays a crucial role in estrogen-mediated signaling pathways and exerts its action as a nuclear transcription factor. Binding of the ligand-activated ERα to the estrogen response element (ERE) is a central part of ERα-associated signal transduction pathways and its aberrant modulation is associated with many disease conditions. Human glutathione S-transferase P1-1 (GSTP) functions as an enzyme in conjugation reactions in drug metabolism and as a regulator of kinase signaling pathways. It is overexpressed in tumors following chemotherapy and has been associated with a poor prognosis in breast cancer. In this study, a novel regulatory function of GSTP has been proposed in which GSTP modulates ERE-mediated ERα signaling events. Ectopic expression of GSTP was able to induce the ERα and ERE-mediated transcriptional activities in ERα-positive but GSTP-negative MCF7 human breast cancer cells. This inductive effect of GSTP on the ERE-transcription activity was diminished when the cells express a mutated form of the enzyme or are treated with a GSTP-specific chemical inhibitor. It was found that GSTP inhibited the expression of the receptor interacting protein 140 (RIP140), a negative regulator of ERα transcription, at both mRNA and protein levels. Our study suggests a novel non-enzymatic role of GSTP which plays a significant role in regulating the classical ERα signaling pathways via modification of transcription cofactors such as RIP140.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 452, Issue 3, 26 September 2014, Pages 840-844
Journal: Biochemical and Biophysical Research Communications - Volume 452, Issue 3, 26 September 2014, Pages 840-844
نویسندگان
Xiyuan Liu, Byoung Ha An, Min Jung Kim, Jong Hoon Park, Young Sook Kang, Minsun Chang,