کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10754035 | 1050346 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential impact of glucose levels and advanced glycation end-products on tubular cell viability and pro-inflammatory/profibrotic functions
ترجمه فارسی عنوان
تأثیر دیفرانسیل سطح گلوکز و محصولات پیشرفته گلیساسیون بر روی سلول های لوله ای و عملکرد پروتئین التهابی / پروبیوتیک
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کلمات کلیدی
GLUTglycated Bovine Serum AlbuminKSFMAGE-BSASGLT2TGF βDIOC6Dulbecco’s Minimum Essential MediumRAGEDHRDMEMBSA - BSANOx - NOXROS - ROSbovine serum albumin - آلبومین سرم گاوinflammation - التهاب( توروم) NADPH oxidase - اکسیداز NADPH Transforming Growth Factor Beta - تبدیل بتا فاکتور رشدGlucose transporter - حمل و نقل گلوکزdihydrorhodamine - دی هیدرو رودامینTubular cells - سلول های لوله ایAge - سنadvanced glycation end-products - محصولات نهایی گلیسایی پیشرفتهDiabetic nephropathy - نفروپاتی دیابتیGlucose - گلوکزReactive oxygen species - گونههای فعال اکسیژن3,3′-dihexyloxacarbocyanine iodide - یدید 3،3'-dihexyloxacarbocyanine
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
High glucose (HG) or synthetic advanced glycation end-products (AGE) conditions are generally used to mimic diabetes in cellular models. Both models have shown an increase of apoptosis, oxidative stress and pro-inflammatory cytokine production in tubular cells. However, the impact of the two conditions combined has rarely been studied. In addition, the impact of glucose level variation due to cellular consumption is not clearly characterized in such experiments. Therefore, the aim of this study was to compare the effect of HG and AGE separately and of both on tubular cell phenotype changes in the HK2 cell line. Moreover, glucose consumption was monitored every hour to maintain the glucose level by supplementation throughout the experiments. We thus observed a significant decrease of apoptosis and H2O2 production in the HK2 cell. HG or AGE treatment induced an increase of total and mitochondrial apoptosis as well as TGF-β release compared to control conditions; however, AGE or HG led to apoptosis preferentially involving the mitochondria pathway. No cumulative effect of HG and AGE treatment was observed on apoptosis. However, a pretreatment with RAGE antibodies partially abolished the apoptotic effect of HG and completely abolished the apoptotic effect of AGE. In conclusion, tubular cells are sensitive to the lack of glucose as well as to the HG and AGE treatments, the AGE effect being more deleterious than the HG effect. Absence of a potential synergistic effect of HG and AGE could indicate that they act through a common pathway, possibly via the activation of the RAGE receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 451, Issue 4, 5 September 2014, Pages 627-631
Journal: Biochemical and Biophysical Research Communications - Volume 451, Issue 4, 5 September 2014, Pages 627-631
نویسندگان
Benoit Franko, Julie Brault, Thomas Jouve, Sylvain Beaumel, Pierre-Yves Benhamou, Philippe Zaoui, Marie José Stasia,