Molecular mechanism regulating myosin and cardiac functions by ELC

Ventricular myosin-S1 (left) consists of a myosin heavy chain (MyHC, light blue) forming the globular motor domain and the a-helical lever arm, which is non-covalently associated with the human ventricular myosin light chain 1(hVLC-1; dark blue) and a regulatory myosin light chain (RLC, turquoise ). Single ventricular myosin molecules showed high stiffness and force generating capacity. Introduction of the E56G mutation into the hVLC-12 (right; hVLC-1E56G) associated with familial hypertrophic cardiomyopathy significantly reduced force myosin stiffness and force generation.