کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10754964 | 1050361 | 2014 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purpurin inhibits adipocyte-derived leucine aminopeptidase and angiogenesis in a zebrafish model
ترجمه فارسی عنوان
پورپورین مهار آمینوپپتیداز لوسین حاصل از آدپیوسیت و آنژیوژنز را در یک مدل سیبفیشی
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کلمات کلیدی
APNHUVECSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAAminopeptidase N - آمینوپپتیداز NAngiogenesis - آنژیوژنزHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانDrug screening - غربالگری مواد مخدرVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Purpurin - پورپورین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Adipocyte-derived leucine aminopeptidase (A-LAP) is a novel member of the M1 family of zinc metallopeptidases, which has been reported to play a crucial role in angiogenesis. In the present study, we conducted a target-based screening of natural products and synthetic chemical libraries using the purified enzyme to search novel inhibitors of A-LAP. Amongst several hits isolated, a natural product purpurin was identified as one of the most potent inhibitors of A-LAP from the screening. In vitro enzymatic analyses demonstrated that purpurin inhibited A-LAP activity in a non-competitive manner with a Ki value of 20Â M. In addition, purpurin showed a strong selectivity toward A-LAP versus another member of M1 family of zinc metallopeptidase, aminopeptidase N (APN). In angiogenesis assays, purpurin inhibited the vascular endothelial growth factor (VEGF)-induced invasion and tube formation of human umbilical vein endothelial cells (HUVEC). Moreover, purpurin inhibited in vivo angiogenesis in zebrafish embryo without toxicity. These data demonstrate that purpurin is a novel specific inhibitor of A-LAP and could be developed as a new anti-angiogenic agent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 450, Issue 1, 18 July 2014, Pages 561-567
Journal: Biochemical and Biophysical Research Communications - Volume 450, Issue 1, 18 July 2014, Pages 561-567
نویسندگان
Hyomi Park, Joong Sup Shim, Beom Seok Kim, Hye Jin Jung, Tae-Lin Huh, Ho Jeong Kwon,