کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10755640 | 1050376 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A mouse model of mitochondrial complex III dysfunction induced by myxothiazol
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کلمات کلیدی
LPSCIIICIVBCS1LRispRieske iron-sulfur proteinblue native gel electrophoresis - الکتروفورز ژل بومی ژلIntraperitoneally - داخل صفاقیlipopolysaccharide - لیپوپلی ساکاریدComplex I - مجتمع IComplex IV - مجتمع IVMouse model - مدل موشMitochondria - میتوکندریاhematoxylin–eosin - هماتوکسیلین ائوزینcomplex III - پیچیده III
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Myxothiazol is a respiratory chain complex III (CIII) inhibitor that binds to the ubiquinol oxidation site Qo of CIII. It blocks electron transfer from ubiquinol to cytochrome b and thus inhibits CIII activity. It has been utilized as a tool in studies of respiratory chain function in in vitro and cell culture models. We developed a mouse model of biochemically induced and reversible CIII inhibition using myxothiazol. We administered myxothiazol intraperitoneally at a dose of 0.56Â mg/kg to C57Bl/J6 mice every 24Â h and assessed CIII activity, histology, lipid content, supercomplex formation, and gene expression in the livers of the mice. A reversible CIII activity decrease to 50% of control value occurred at 2Â h post-injection. At 74Â h only minor histological changes in the liver were found, supercomplex formation was preserved and no significant changes in the expression of genes indicating hepatotoxicity or inflammation were found. Thus, myxothiazol-induced CIII inhibition can be induced in mice for four days in a row without overt hepatotoxicity or lethality. This model could be utilized in further studies of respiratory chain function and pharmacological approaches to mitochondrial hepatopathies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 446, Issue 4, 18 April 2014, Pages 1079-1084
Journal: Biochemical and Biophysical Research Communications - Volume 446, Issue 4, 18 April 2014, Pages 1079-1084
نویسندگان
Mina Davoudi, Jukka Kallijärvi, Sanna Marjavaara, Heike Kotarsky, Eva Hansson, Per Levéen, Vineta Fellman,