کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10755926 | 1050378 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Integrin α1β1 participates in chondrocyte transduction of osmotic stress
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کلمات کلیدی
PBSDMEMDICCTRLTRPV4Integrin α1β1Intracellular calcium transientsDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده Dulbecco[Ca2+]i - [Ca2 +] iOsmolarity - اسمولاریسمPhosphate buffered saline - فسفات بافر شورIntracellular calcium - کلسیم داخل سلولیdifferential interference contrast - کنتراست تداخل دیفرانسیلControl - کنترلChondrocytes - کندروسیت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Deletion of the integrin α1 subunit inhibited the ability of chondrocytes to respond to a hypo-osmotic stress with [Ca2+]i transients ex vivo and in vitro. The percentage of chondrocytes responding ex vivo was smaller than in vitro and of the cells that responded, more single [Ca2+]i transients were observed ex vivo compared to in vitro. Immunocytochemistry confirmed the presence of TRPV4 on wildtype and integrin α1-null chondrocytes, however application of GSK101 revealed that TRPV4 could be activated on wildtype but not integrin α1-null chondrocytes. Integrin α1β1 is a key participant in chondrocyte transduction of a hypo-osmotic stress. Furthermore, the mechanism by which integrin α1β1 influences osmotransduction is independent of matrix binding, but likely dependent on the chondrocyte osmosensor TRPV4.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 445, Issue 1, 28 February 2014, Pages 184-190
Journal: Biochemical and Biophysical Research Communications - Volume 445, Issue 1, 28 February 2014, Pages 184-190
نویسندگان
Christina L. Jablonski, Samuel Ferguson, Ambra Pozzi, Andrea L. Clark,