کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10756093 | 1050381 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mutant OmpF porins of Yersinia pseudotuberculosis with deletions of external loops: Structure-functional and immunochemical properties
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Mutant OmpF porins of Yersinia pseudotuberculosis with deletions of external loops: Structure-functional and immunochemical properties Mutant OmpF porins of Yersinia pseudotuberculosis with deletions of external loops: Structure-functional and immunochemical properties](/preview/png/10756093.png)
چکیده انگلیسی
Recombinant mutant OmpF porins from Yersinia pseudotuberculosis outer membrane were obtained using site-directed mutagenesis. Here we used four OmpF mutants where single extracellular loops L1, L4, L6, and L8 were deleted one at a time. The proteins were expressed in Escherichia coli at levels comparable to full-sized recombinant OmpF porin and isolated from the inclusion bodies. Purified trimers of the mutant porins were obtained after dialysis and consequent ion-exchange chromatography. Changes in molecular and spatial structure of the mutants obtained were studied using SDS-PAGE and optical spectroscopy (circular dichroism and intrinsic protein fluorescence). Secondary and tertiary structure of the mutant proteins was found to have some features in comparison with that of the full-sized recombinant OmpF. As shown by bilayer lipid membrane technique, the pore-forming activity of purified mutant porins was identical to OmpF porin isolated from the bacterial outer membrane. Lacking of the external loops mentioned above influenced significantly upon the antigenic structure of the porin as demonstrated using ELISA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 445, Issue 2, 7 March 2014, Pages 428-432
Journal: Biochemical and Biophysical Research Communications - Volume 445, Issue 2, 7 March 2014, Pages 428-432
نویسندگان
O.V. Sidorova, V.A. Khomenko, O. Yu Portnyagina, G.N. Likhatskaya, T.I. Vakorina, N. Yu Kim, D.K. Chistyulin, T.F. Solov'eva, O.D. Novikova,