کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10756178 | 1050382 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Steroid hormones are novel nucleoside transport inhibitors by competition with nucleosides for their transporters
ترجمه فارسی عنوان
هورمون های استروئیدی نوترکیب مهار کننده های نوکلئوزیدی هستند که رقابت با نوکلئوزید ها برای حمل کنندگان آنها است
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کلمات کلیدی
PBSNBTIDMEMFBSENTCNTBSA - BSAbovine serum albumin - آلبومین سرم گاوEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدEstradiol - استرادیولequilibrative nucleoside transporter - ترانسفورماتور نوکلئوتیدی تعادلnucleoside transporter - حمل و نقل نوکلئیدیconcentrative nucleoside transporter - حمل کننده نوکلئوز غلیظfetal bovine serum - سرم جنین گاوDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoSteroid hormone - هورمون استروئیدProgesterone - پروژسترون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Nucleoside transport is important for nucleic acid synthesis in cells that cannot synthesize nucleosides de novo, and for entry of many cytotoxic nucleoside analog drugs used in chemotherapy. This study demonstrates that various steroid hormones induce inhibition of nucleoside transport in mammalian cells. We analyzed the inhibitory effects of estradiol (E2) on nucleoside transport using SH-SY5Y human neuroblastoma cells. We observed inhibitory effects after acute treatment with E2, which lasted in the presence of E2. However, when E2 was removed, the effect immediately disappeared, suggesting that E2 effects are not mediated through the canonical regulatory pathway of steroid hormones, such as transcriptional regulation. We also discovered that E2 could competitively inhibit thymidine uptake and binding of the labeled nucleoside transporter inhibitor, S-[4-nitrobenzyl]-6-thioinosine (NBTI), indicating that E2 binds to endogenous nucleoside transporters, leading to inhibition of nucleoside transport. We then tested the effects of various steroids on nucleoside uptake in NBTI-sensitive cells, SH-SY5Y and NBTI-insensitive cells H9c2 rat cardiomyoblasts. We found E2 and progesterone clearly inhibited both NBTI-sensitive and insensitive uptake at micromolar concentrations. Taken together, we concluded that steroid hormones function as novel nucleoside transport inhibitors by competition with nucleosides for their transporters.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 2, 10 January 2014, Pages 505-510
Journal: Biochemical and Biophysical Research Communications - Volume 443, Issue 2, 10 January 2014, Pages 505-510
نویسندگان
Masahiro Kaneko, Fumihiko Hakuno, Hiroyasu Kamei, Daisuke Yamanaka, Kazuhiro Chida, Shiro Minami, Imogen R. Coe, Shin-Ichiro Takahashi,