Identification of an atypical calcium-dependent calmodulin binding site on the C-terminal domain of GluN2A

- Calmodulin was identified by MS as a GluN2A C-terminus-associated protein.
- Ca2 +/calmodulin bound directly to GluN2A with high affinity (5.2 ± 2.4 nM) in vitro.
- Disruption of classic Ca2+/calmodulin recognition motifs did not affect binding.
- Trp-1014 of GluN2A was a critical determinant of Ca2+/calmodulin binding in vitro.