Direct interaction between EFL1 and SBDS is mediated by an intrinsically disordered insertion domain

- The interaction between EFL1 and SBDS was elucidated.
- EFL1 binds directly to SBDS with an association constant of 1.27 × 107 M−1.
- The interaction is governed by the insertion domain of EFL1 and domain II-III of SBDS.
- The insertion domain of EFL1 acts as an intrinsically disordered domain.
- Roles of each domain of EFL1 and SBDS in Tif6 release are proposed.