کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10756630 | 1050387 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
VEGF111b, a new member of VEGFxxxb isoforms and induced by mitomycin C, inhibits angiogenesis
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کلمات کلیدی
FBSVEGF-RVEGFR-2RT-PCRHUVECSHRPDSSPSSreverse transcriptase-PCR - RT-PCR معکوسfetal bovine serum - سرم جنین گاوHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانAnti-Angiogenesis - ضد آنژیوژنزVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)splice variant - نوع اتصالاتHorseradish peroxidase - پراکسیداز هوررادیشVEGF receptor - گیرنده VEGF
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: VEGF111b, a new member of VEGFxxxb isoforms and induced by mitomycin C, inhibits angiogenesis VEGF111b, a new member of VEGFxxxb isoforms and induced by mitomycin C, inhibits angiogenesis](/preview/png/10756630.png)
چکیده انگلیسی
Vascular endothelial growth factor (VEGF-A) stimulating angiogenesis is required for tumor growth and progression. The conventional VEGF-A isoforms have been considered as pro-angiogenic factors. Another family of VEGF-A isoforms generated by alternative splicing, termed VEGFxxxb isoforms, has anti-angiogenic property, exemplified by VEGF165b. Here, we identify a new number of VEGFxxx family-VEGF111b induced by mitomycin C, although not detected in mitomycin C-unexposed ovarian cancer cells. SKOV3 cells were transfected with pcDNA3.1 empty vector, pcDNA3.1-VEGF111b or pcDNA3.1-VEGF165b to collect conditioned mediums respectively. VEGF111b overexpression inhibits proliferation, migration and tube formation of endothelial cell by inhibiting VEGF-R2 phosphorylation and its downstream signaling, similar to VEGF165b but slightly lower than VEGF165b. The anti-angiogenic property depends on the six amino acids of exon 8b of the VEGFxxxb isoforms. Our results show that VEGF111b is a novel potent anti-angiogenic agent that can target the VEGF-R2 and its signaling pathway to inhibit ovarian tumor growth.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 441, Issue 1, 8 November 2013, Pages 18-24
Journal: Biochemical and Biophysical Research Communications - Volume 441, Issue 1, 8 November 2013, Pages 18-24
نویسندگان
Fang Gu, Xiuli Li, Jian Kong, Bing Pan, Min Sun, Lemin Zheng, Yuanqing Yao,