کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10756962 | 1050390 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Anti-platelet activity of a three-finger toxin (3FTx) from Indian monocled cobra (Naja kaouthia) venom
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کلمات کلیدی
FITCRP-HPLCADPGPCRG-protein coupled receptor - G-پروتئین همراه گیرندهAdenosine di-phosphate - آدنوزین دی فسفاتSPR - تشدید پلاسمون سطحیSurface Plasma Resonance - رزونانس پلاسما سطحAnti-platelet - ضد پلاکتfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتMolecular weight - وزن مولکولیReversed phase high performance liquid chromatography - کروماتوگرافی مایع با عملکرد بالا فاز برگشت پذیرP2Y receptor - گیرنده P2Y
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Anti-platelet activity of a three-finger toxin (3FTx) from Indian monocled cobra (Naja kaouthia) venom Anti-platelet activity of a three-finger toxin (3FTx) from Indian monocled cobra (Naja kaouthia) venom](/preview/png/10756962.png)
چکیده انگلیسی
A low molecular weight anti-platelet peptide (6.9Â kDa) has been purified from Naja kaouthia venom and was named KT-6.9. MALDI-TOF/TOF mass spectrometry analysis revealed the homology of KT-6.9 peptide sequence with many three finger toxin family members. KT-6.9 inhibited human platelet aggregation process in a dose dependent manner. It has inhibited ADP, thrombin and arachidonic acid induced platelet aggregation process in dose dependent manner, but did not inhibit collagen and ristocetin induced platelet aggregation. Strong inhibition (70%) of the ADP induced platelet aggregation by KT-6.9 suggests competition with ADP for its receptors on platelet surface. Anti-platelet activity of KT-6.9 was found to be 25 times stronger than that of anti-platelet drug clopidogrel. Binding of KT-6.9 to platelet surface was confirmed by surface plasma resonance analysis using BIAcore X100. Binding was also observed by a modified sandwich ELISA method using anti-KT-6.9 antibodies. KT-6.9 is probably the first 3FTx from Indian monocled cobra venom reported as a platelet aggregation inhibitor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 441, Issue 3, 22 November 2013, Pages 550-554
Journal: Biochemical and Biophysical Research Communications - Volume 441, Issue 3, 22 November 2013, Pages 550-554
نویسندگان
Chandrasekhar Chanda, Angshuman Sarkar, Srinivas Sistla, Dibakar Chakrabarty,